Journal: eLife

Article Title: Oxytocin signaling in the posterior hypothalamus prevents hyperphagic obesity in mice

doi: 10.7554/eLife.75718

Figure Lengend Snippet: ( A ) Schematic of the virus injection. AAV-Cre or vehicle was injected into the bilateral PVH of Oxt flox/– male mice. ( B ) Representative coronal sections of the PVH from Oxt flox/– mice received vehicle (left) or AAV-Cre (right) injection. Data were obtained at 5 weeks after the injection. Magenta and green represent Oxt and Cre in situ stainings, respectively. Blue, DAPI. Scale bar, 50 μm. ( C ) The number of remaining Oxt + neurons in the PVH of mice that received AAV-Cre injection. **p<0.01, one-way ANOVA with post hoc Tukey’s HSD. N=5 each. ( D ) Time course of body weight after AAV-Cre or vehicle injection. N=6 each. ( E ) Representative photos of Oxt flox/– mice that received either vehicle (left) or AAV-Cre injection (right). Five weeks after the injection. Scale bar, 5 cm. ( F ) Body weight of wild-type ( +/+ ), Oxt KO ( –/– ), and Oxt cKO ( flox/– ) mice. The weight was measured at 5 weeks after injection of either vehicle or AAV-Cre . Note that this time point corresponds to 13 weeks of age. **p<0.01, one-way ANOVA with post hoc Tukey’s HSD. N=10, 7, 9, and 10 for +/+ , –/– , flox/– vehicle, and flox/– Cre, respectively. ( G ) Relationship between the number of remaining Oxt + neurons in the PVH and the body weight of Oxt flox/– mice shown in ( F ). Magenta, exponential fit for the data from both Cre and vehicle. ( H ) Time course of daily food intake, defined as the average food intake in each week after AAV-Cre or vehicle injection. ***p<0.001, Student’s t -test with post hoc Bonferroni correction. N=6 each. ( I ) Cumulative food intake during the 5 weeks after the injection. ***p<0.001, Student’s t -test. N=6 each. Error bars, standard error of mean (SEM).

Article Snippet: In and , exponential fit was calculated by Igor (WaveMetrics).

Techniques: Virus, Injection, In Situ

Journal: eLife

Article Title: Oxytocin signaling in the posterior hypothalamus prevents hyperphagic obesity in mice

doi: 10.7554/eLife.75718

Figure Lengend Snippet: ( A ) Schematic of the virus injection. AAV-Cre or vehicle was injected into the bilateral SO of Oxt flox/– male mice. ( B ) Representative coronal sections of left SO from Oxt flox/– mice received vehicle (left) or AAV-Cre (right) injection. Five weeks after the injection. Magenta and green represent Oxt and Cre in situ stainings, respectively. Blue, DAPI. Scale bar, 50 μm. ( C ) The number of remaining Oxt + neurons in the SO of mice that received AAV-Cre injection. **p<0.01, one-way ANOVA with post hoc Tukey’s HSD. N=5 each. ( D ) The body weight of Oxt flox/– mice did not differ between vehicle or AAV-Cre (Student’s t -test). N=6 each. Data were obtained at 5 weeks after the injection. ( E ) Relationship between the number of remaining Oxt + neurons in the SO and the body weight of Oxt flox/– mice shown in ( D ). Magenta, exponential fit for the data from both Cre and vehicle. ( F ) The time course of daily food intake was not statistically different (Student’s t -test with post hoc Bonferroni correction). N=6 each. ( G ) Cumulative food intake during the 5 weeks after the injection. N=6 each. Error bars, SEM.

Article Snippet: In and , exponential fit was calculated by Igor (WaveMetrics).

Techniques: Virus, Injection, In Situ